PAPER & PATENT

Takashi Okumura, Yumi Horie, Chen-Yi Lai, Huan-Ting Lin, Hirofumi Shoda, Bunki Natsumoto; Keishi Fujio; Eri Kumaki; Tsubasa Okano; Shintaro Ono; Kay Tanita, Tomohiro Morio, Hirokazu Kanegane, Hisanori Hasegawa, Fumitaka Mizoguchi, Kimito Kawahata, Hitoshi Kohsaka, Hiroshi Moritake, Hiroyuki Nunoi, Hironori Waki, Shin-ichi Tamaru, Takayoshi Sasako, Toshimasa Yamauchi, Takashi Kadowaki, Hiroyuki Tanaka, Sachiko Kitanaka, Ken Nishimura, Manami Ohtaka, Mahito Nakanishi and Makoto Otsu.
Robust and highly efficient hiPSC generation from patient non-mobilized peripheral blood-derived CD34+ cells using the auto-erasable Sendai virus vector.
Stem Cell Research & Therapy, 10(1), 185. (2019)

Ling Li, Jee Hoon Roh, Hee Jin Kim, Hyun Jung Park, Minchul Kim, Wonyoung Koh, Hyohoon Heo, Jong Wook Chang, Mahito Nakanishi, Taeyoung Yoon, Duk L. Na and Jihwan Song.
The First Generation of iPSC Line from a Korean Alzheimer’s Disease Patient Carrying APP-V715M Mutation Exhibits a Distinct Mitochondrial Dysfunction.
Experimental Neurobiology, in press. (2019)

Koichiro Nishino, Yoshikazu Arai, Ken Takasawa, Masashi Toyoda, Mayu Yamazaki-Inoue, Tohru Sugawara, Hidenori Akutsu, Ken Nishimura, Manami Ohtaka, Mahito Nakanishi and Akihiro Umezawa.
Epigenetic-scale comparison of human iPSCs generated by retrovirus, Sendai virus or episomal vectors.
Regenerative Therapy, 9, 71-78. (2018)

Atsutaka Minagawa, Toshiaki Yoshikawa, Masaki Yasukawa, Akitsu Hotta, Mihoko Kunitomo, Shoichi Iriguchi, Maiko Takiguchi, Yoshiaki Kassai, Eri Imai, Yutaka Yasui, Yohei Kawai, Rong Zhang, Yasushi Uemura, Hiroyuki Miyoshi, Mahito Nakanishi, Akira Watanabe, Akira Hayashi, Kei Kawana and Shin Kaneko.
Enhancing T Cell Receptor Stability in Rejuvenated iPSC-Derived T Cells Improves Their Use in Cancer Immunotherapy.
Cell Stem Cell, 23, 850-858. (2018).

Ling Li, Jee Hoon Roh, Eun Hyuk Chang, Yoonkyung Lee, Suji Lee, Minchul Kim, Wonyoung Koh, Jong Wook Chang, Hee Jin Kim, Mahito Nakanishi, Roger A. Barker, Duk L. Na and Jihwan Song
iPSC Modeling of Presenilin1 Mutation in Alzheimer’s Disease with Cerebellar Ataxia.
Experimental Neurobiology, 27, 350-364. (2018).

Masaya Tsukamoto, Toshiya Nishimura, Kyohei Yodoe, Ryoji Kanegi, Yasunori Tsujimoto, Md Emtiaj Alam, Mizuki Kuramochi, Mitsuru Kuwamura, Manami Ohtaka, Ken Nishimura, Mahito Nakanishi, Toshio Inaba, Kikuya Sugiura. and Shingo Hatoya.
Generation of Footprint-Free Canine Induced Pluripotent Stem Cells Using Auto-Erasable Sendai Virus Vector.
Stem Cells and Development, 27, 1577-1586. (2018).

Suji Lee, Ji Young Huh, David M. Turner, Soohyeon Lee, James Robinson, Jeremy E. Stein, Sung Han Shim, Chang Pro Hong, Myung Seo Kang, Masato Nakagawa, Shin Kaneko, Mahito Nakanishi, Mahendra S. Rao, Andreas Kurtz, Glyn N. Stacey, Steven G.E. Marsh, Mark L. Tuner and Jihwan Song
Repurposing the Cord Blood Bank for Haplobanking of HLA-Homozygous iPSCs and Their Usefulness to Multiple Populations.
Stem Cells, 36, 1552-1566. (2018).

Kenji Kubara, Kazuto Yamazaki, Yasuhara Ishihara, Takuya Naruto, Huan-Ting Li, Ken Nishimura, Manami Ohtaka, Mahito Nakanishi, Masashi Ito, Kappei Tsukahara, Tomohiro Morio, Masatoshi Takagi and Makoto Otsu
Status of KRAS in iPSCs Impacts upon Self-Renewal and Differentiation Propensity.
Stem Cell Reports, 11, 1-15. (2018).

Norihiro Ueda, Yasushi Uemura, Rong Zhang, Schuichi Kitayama, Shoichi Iriguchi,
Yohei Kawai, Yutaka Yasui, Minako Tatsumi, Tatsuki Ueda, Tian-Yi Liu, Yasutaka Mizoro, Chihiro Okada, Akira Watanabe, Mahito Nakanishi, Satoru Senju, Yasuharu Nishimura, Kiyotaka Kuzushima, Hitoshi Kiyoi, Tomoki Naoe and Shin Kaneko
Generation of TCR-Expressing Innate Lymphoid-like Helper Cells that Induce Cytotoxic T Cell-Mediated Anti-leukemic Cell Response.
Stem Cell Reports, 10, 1935-1946. (2018).

Akiko Saito, Akio Ooki, Takashi Nakamura, Shoko Onodera, Kamichika Hayashi, Daigo Hasegawa, Takahito Okudaira, Katsuhito Watanabe, Hiroshi Kato, Takeshi Onda, Akira Watanabe, Kenjiro Kosaki, Ken Nishimura, Manami Ohtaka, Mahito Nakanishi, Teruo Sakamoto, Akira Yamaguchi, Kenji Sueishi, and Toshifumi Azuma
Targeted reversion of induced pluripotent stem cells from patients with human cleidocranial dysplasia improves bone regeneration in rat calvarial bone defect model.
Stem Cell Research & Therapy, 9, 12. (2018).

Jacek Stepniewski, Tomasz Pacholczak, Aniela Skrzypczyk, Maciej Ciesla, Agata Szade, Krzysztof Szade, Romain Bidanel, Agnieszka Langrzyk, Radoslaw Grochowski, Felix Vandermeeren, Neli Kachamakova-Trojanowska, Mateusz Jez, Grazyna Drabik, Mahito Nakanishi, Alicja Jozkowicz and Jozef Dulak
Heme oxygenase-1 affects generation and spontaneous cardiac differentiation of induced pluripotent stem cells.
IUBMB Life, 70, 129-142. (2018).

Daigo Hasegawa, Hiromi Ochiai-Shino, Shoko Onodera, Takeshi Nakamura, Akiko Saito, Takeshi Onda, Katsuhito Watanabe, Ken Nishimura, Manami Ohtaka, Mahito Nakanishi, Kenjiro Kosaki, Akira Yamaguchi, Takashi Shibahara and Toshifumi Azuma
Gorlin syndrome-derived induced pluripotent stem cells are hypersensitive to hedgehog-mediated osteogenic induction.
PLoS One, 12, e0186879. (2017).

Julio Castano, Clara Bueno, Senda Jimenez-Delago, Heleia Roca-Ho, Mario F. Fraga, Agustin F. Fernandez, Mahito Nakanishi, Raul Torres-Ruiz, Sandra Rodriguez-Perales and Pablo Menendez
Generation and characterization of a human iPSC cell line expressing inducible Cas9 in the “safe harbor” AAVS1 locus.
Stem Cell Research, 21, 137-140. (2017).

Ken Nishimura, Manami Ohtaka, Hitomi Takada, Akira Kurisaki, Nhi Vo Kieu Trana, Yen Thi Hai Trana, Koji Hisatakea, Masayuki Sano and Mahito Nakanishi
Simple and effective generation of transgene-free induced pluripotent stem cells using an auto-erasable Sendai virus vector responding to microRNA-302.
Stem Cell Research, 23, 13-19. (2017).

Mana Iizuka-Koga, Hiromitsu Asashima, Miki Ando, Chen-Yi Lai, Shinji Mochizuki, Mahito Nakanishi, Toshinobu Nishimura, Hiroto Tsuboi, Tomoya Hirota, Hiroyuki Takahashi, Isao Matsumoto, Makoto Otsu, and Takayuki Sumida
Functional analysis of dendritic cells generated from T-iPS cells from CD4+ T cell clones of Sjögren’s syndrome.
Stem Cell Reports, 8, 1155-1163. (2017).

Ken Nishimura, Shiho Aizawa, Fransiska Liliani Nugroho, Emi Shiomitsu, Tran Thi Hai Yen, Bui Phuong Linh, Borisova Dmitrievna Evgeniia, Yuta Sakuragi, Hitomi Takada, Akira Kurisaki, Yohei Hayashi, Aya Fukuda, Mahito Nakanishi, Koji Hisatake
A role for KLF4 in promoting the metabolic shift via CL1 during induced pluripotent stem cell generation.
Stem Cell Reports, 8, 787-801 (2017).

Alvaro Muñoz-López, Damia Romero-Moya, Cristina Prieto, Verónica Ramos-Mejía, Antonio Agraz-Doblas, Ignacio Varela, Marcus Buschbeck, Anna Palau, Xonia Carvajal-Vergara, Alessandra Giorgetti, Anthony Ford, Majlinda Lako, Isabel Granada, Neus Ruiz-Xivillé, Sandra Rodríguez-Perales, Raul Torres-Ruíz, Ronald W Stam, Jose Luis Fuster, Mario. F. Fraga, Mahito Nakanishi, Gianni Cazzaniga, Michela Bardini, Isabel Cobo, Gustavo F Bayon, Agustín Fernández, Clara Bueno and Pablo Menendez
Development refractoriness of MLL-rearranged human B-cell acute leukemias to reprogramming into pluripotency.
Stem Cell Reports, 7, 602-618. (2016).

Kohji Okamura, Hironari Sakaguchi, Rie Sakamoto, Mahito Nakanishi, Ken Nishimura, Mayu Yamazaki-Inoue, Manami Ohtaka, Vaiyapuri Periasamy, Ali Alshatwi, Akon Higuchi, Kazunori Hanaoka, Kazuhiko Nakabayashi, Shuji Takada, Kenichiro Hata, Masashi Toyoda, and Akihiro Umezawa
Distinctive features of single nucleotide alterations in induced pluripotent stem cells with different types of DNA repair deficiency disorders.
Scientific Reports, 6, 26342. (2016).

Kimihiko Banno, Sayaka Omori, Katsuya Hirata, Nobutoshi Nawa, Natsuki Nakagawa, Ken Nishimura, Manami Ohtaka, Mahito Nakanishi, Tetsushi Sakuma, Takashi Yamamoto, Toshiyuki Yamamoto, Chikara Kokubu, Junji Takeda, Hidetoshi Taniguchi, Hitomi Arahori, Kazuko Wada, Yasuji Kitabatake and Keiichi Ozono
Systematic Cellular Disease Models Reveal Synergistic Interactions of Trisomy 21 and GATA1 Mutations on Hematopoietic Abnormalities.
Cell Reports, 15, 1228-1241. (2016).

Ruxandra Dafinica, Jakub Scaber, Nidaa Ababneh, Tatjana Lalic, Gregory Weir, Jane Vowles, Andrew G. L. Douglas, Alexandra Fletcher-Jones, Cathy Browne, Mahito Nakanishi, Martin R. Turner, Richard Wade-Martins, Sally A Cowley and Kevin Talbot
C9orf72 Hexanucleotide Expansions are Associated with Altered ER Calcium Homeostasis and Stress Granule Formation in iPSC-Derived Neurons from Patients with Amyotrophic Lateral Sclerosis and Frontotemporal Dementia.
Stem Cells, 134, 2063-2078. (2016).

Takuya Matsumoto, Koki Fujimori, Tomoko Andoh-Noda, Takayuki Ando, Naoko Kuzumaki, Manabu Toyoshima, Hirobumi Tada, Kent Imaizumi, Mitsuru Ishikawa, Ryo Yamaguchi, Miho Isoda, Zhi Zhou, Shigeto Sato, Tetsuro Kobayashi, Manami Ohtaka, Ken Nishimura, Hiroshi Kurosawa, Takeo Yoshikawa, Takuya Takahashi, Mahito Nakanishi, Manabu Ohyama, Nobutaka Hattori, Wado Akamatsu and Hideyuki Okano
Functional Neurons Generated from T Cell-Derived Induced Pluripotent Stem Cells for Neurological Disease Modeling.
Stem Cell Reports, 6, 422-435. (2016).

Álvaro Muñoz-López, Eddy. H. J. van Roon, Ronald Stam, Dolors Colomer, Mahito Nakanishi, Clara Bueno & Pablo Menendez
Cellular ontogeny and hierarchy influences the reprogramming efficiency of human B-cells into induced pluripotent stem cells.
Stem Cells, 34, 581-587. (2016).

Adam E. Handel, Satyan Chintawar, Tatjana Lalic, Emma Whiteley, Jane Vowles, Alice Giustacchini, Karene Argoud, Paul Sopp, Mahito Nakanishi, Rory Bowden, Sally Cowley, Sarah Newey, Colin Akerman, Chris P. Ponting and M. Zameel Cader
Assessing similarity to primary tissue and cortical layer identity in induced pluripotent stem cell-derived cortical neurons through single cell transcriptomics.
Human Molecular Genetics, 25, 989-1000. (2016).

Shuichi Kitayama, Rong Zhang, Tian-Yi Liu, Norihiro Ueda, Shoichi Iriguchi, Yutaka Yasui, Yohei Kawai, Minako Tatsumi, Norihito Hirai, Yasutaka Mizoro, Tatsuaki Iwama, Akira Watanabe, Mahito Nakanishi, Kiyotaka Kuzushima, Yasushi Uemura and Shin Kaneko
Cellular adjuvant properties and direct cytotoxicity of redifferentiated Vα24 invariant human NKT-like cells from iPS cells.
Stem Cell Reports, 6, 213-227. (2016).

Aija Kyttälä, Roksana Moraghebi, Cristina Valensisi, Johannes Kettunen, Colin Andrus, Kalyan Kumar Pasumarthy, Mahito Nakanishi, Ken Nishimura, Manami Ohtaka, Jere Weltner, Ben Van Handel, Olavi Parkkonen, Juha Sinisalo, Anu Jalanko, R. David Hawkins, Niels-Bjarne Woods, Timo Otonkoski and Ras Trokovic
Genetic variability overrides the impact of parental cell-type and determines iPSCs differentiation potential.
Stem Cell Reports, 6, 200-212. (2016).

Sachiko Yamasaki, Atsuko Hamada, Eri Akagi, Hirotaka Nakatao, Manami Ohtaka, Ken Nishimura, Mahito Nakanishi, Shigeaki Toratani and Tetsuji Okamoto
Generation and maintenance of Cleidocranial dysplasia-specific human induced pluripotent stem cells in complete serum-, feeder-, and integration-free culture.
In Vitro Cellular & Developmental Biology, 52, 252-264. (2016).

Clara Bueno, Jose Luis Sardina, Bruno Di Stefano, Damia Romero-Moya, Alvaro Muñoz-López, Lorena Ariza, María Carmen Chillón, Ana Balanzategui, Julio Castaño, Antonio Herreros, Mario. F. Fraga, Agustín Fernández, Isabel Granada, Oscar Quintana-Bustamante, Jose Carlos Segovia, Ken Nishimura, Manami Ohtaka, Mahito Nakanishi, Thomas Graf and Pablo Menendez
Reprogramming human B-cells into induced pluripotent stem cells and its enhancement by C/EBPα.
Leukemia, 30, 674-682. (2016).

Huan-Ting Lin, Makoto Otsu, Hideki Masaki, Tomoyuki Yamaguchi, Taizo Wada, Akihiro Yachie, Ken Nishimura, Manami Ohtaka, Mahito Nakanishi and Hiromitsu Nakauchi
An assessment of the effects of ectopic gp91phox expression in XCGD iPSC-derived neutrophils.
Molecular Therapy – Methods and Clinical Development, 2, 15046. (2015).

Miki Ando, Toshinobu Nishimura, Satoshi Yamazaki, Tomoyuki Yamaguchi, Ai Kawana-Tachikawa, Tomonari Hayama, Yusuke Nakauchi, Jun Ando, Yasunori Ota, Satoshi Takahashi, Ken Nishimura, Manami Ohtaka, Mahito Nakanishi, John J Miles, Scott R Burrows, Malcolm K Brenner and Hiromitsu Nakauchi
A Safeguard System for Induced Pluripotent Stem Cell-Derived Rejuvenated T Cell Therapy.
Stem Cell Reports, 5, 597-608. (2015).

Nikolett M. Biel, Katherine E. Santostefano, Bayli B. DiVita, Nihal El Rouby, Santiago D. Carrasquilla, Chelsey Simmons, Mahito Nakanishi, Rhonda M. Cooper-DeHoff, Julie A. Johnson and Naohiro Terada
Vascular smooth muscle cells from hypertensive patient-derived induced pluripotent stem cells to advance hypertension pharmacogenomics.
STEM CELLS Translational Medicine, 4, 1380-1390. (2015).

Hideki Masaki, Megumi Kato-Itho, Ayumi Umino, Hideyuki Sato, Sanae Hamanaka, Toshihiro Kobayashi, Tomoyuki Yamaguchi, Ken Nishimura, Minami Ohtaka, Mahito Nakanishi and Hiromitsu Nakauchi
Interspecific in vitro assay for chimera-forming ability of human pluripotent stem cells.
Development, 142, 3222-3230. (2015).

Kazuo Takayama, Yuta Morisaki, Shuichi Kuno, Yasuhito Nagamoto, Kazuo Harada, Norihisa Furukawa, Manami Ohtaka, Ken Nishimura, Kazuo Imagawa, Fuminori Sakurai, Masashi Tachibana, Ryo Sumazaki, Emiko Noguchi, Mahito Nakanishi, Kazumasa Hirata, Kenji Kawabata, and Hiroyuki Mizuguchi
Prediction of interindividual differences in hepatic functions and drug sensitivity by using human iPS-derived hepatocytes.
Proc. Natl. Acad. Sci. USA, 111, 16772-16777. (2014).

Ras Trokovic, Jere Weltner, Ken Nishimura, Manami Ohtaka, Mahito Nakanishi, Veikko Salomaa, Anu Jalanko, Timo Otonkoski and Aija Kyttälä
Advanced Feeder-Free Generation of iPSC Directly From Blood Cells.
STEM CELLS Translational Medicine, 3, 1402-1409. (2014).

Ken Nishimura, Tetsuo Kato, Lalhaba Oinam, Emi Shiomitsu, Daisuke Ayakawa, Manami Ohtaka, Aya Fukuda, Mahito Nakanishi, Koji Hisatake
Manipulation of Klf4 expression generates paused iPSCs stalled at distinct stages of reprogramming.
Stem Cell Reports, 3, 915-929. (2014).

Shiho Kawagoe, Takashi Higuchi, Manami Otaka, Yota Shimada, Hiroshi Kobayashi, Hiroyuki Ida, Tohya Ohashi, Hironao James Okano, Mahito Nakanishi and Yoshikatsu Eto
Morphological features of iPS cells generated from Fabry disease skin fibroblasts using Sendai virus vector (SeVdp).
Molecular Genetics and Metabolism, 109, 386-389. (2013).

Hiroshi Wakao, Kazunori Yoshikiyo, Uichi Koshimizu, Tomoko Furukawa, Kei Enomoto, Tomomi Matsunaga, Tomofumi Tanaka, Yusuke Yasutomi, Takashi Yamada, Hisanori Minakami, Jyunji Tanaka, Atsushi Oda, Tomoyuki Sasaki , Rika Wakao, Olivier Lantz, Tadashi Udagawa, Yukie Sekiya, Kazue Higuchi, Nobuyuki Harada, Manami Ohtaka, Mahito Nakanishi　and Hiroyoshi Fujita
Expansion of functional human mucosal-associated invariant T cells via reprogramming to pluripotency and redifferentiation.
Cell Stem Cell, 12, 546-558. (2013).

Toshinobu Nishimura, Shin Kaneko, Ai Kawana-Tachikawa, Yoko Tajima, Haruo Gotoh, Dayong Zhu, Kaori Nakayama, Shoichi Iriguchi, Takafumi Shimizu, Naoya Takayama, Daisuke Yamada, Ken Nishimura, Manami Ohtaka, Nobukazu Watanabe, Satoshi Takahashi, Aikichi Iwamoto, Haruhiko Koseki, Mahito Nakanishi, Koji Eto and Hiromitsu Nakauchi
Regeneration of HIV-1 Patient’s Antigen-specific T Cells by Reprogramming and Re-differentiation.
Cell Stem Cell, 12, 114-126. (2013).

Mahito Nakanishi and Makoto Otsu
Development of Sendai virus vectors and their potential applications in gene therapy and regenerative medicine.
Current Gene Therapy, 12, 410-416. (2012).

Ken Nishimura, Masayuki Sano, Manami Ohtaka, Birei Furuta, Yoko Umemura, Yoshihiro Nakajima, Yuzuru Ikehara, Toshihiro Kobayashi, Hideyuki Sato, Hiroaki Segawa, Satoko Takayasu, Kaori Motomura, Eriko Uchida, Toshie Kanayasu-Toyoda, Makoto Asashima, Hiromitu Nakauchi, Teruhide Yamaguchi and Mahito Nakanishi
Development of defective and persistent Sendai virus vector: a unique gene delivery/expression system ideal for cell reprogramming.
J. Biol. Chem., 286, 4760-4771. (2011).

Naoya Takayama, Satoshi Nishimura, Sou Nakamura, Takafumi Shimizu, Ryoko Ohnishi, Hiroshi Endo, Tomoyuki Yamaguchi, Makoto Otsu, Ken Nishimura, Mahito Nakanishi, Akira Sawaguchi, Ryozo Nagai, Kazutoshi Takahashi, Shinya Yamanaka, Hiromitsu Nakauchi and Koji Eto
Transient activation of c-Myc expression is critical for efficient platelet generation from human induced pluripotent stem cells.
J. Exp. Med., 207, 2817-2830. (2010).